Visualization of Cytoskeletal Elements by the Atomic Force Microscope

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dc.creatorBerdyyeva, T.
dc.creatorWoodworth, C. D.
dc.creatorSokolov, I.
dc.date2004-06-26
dc.date.accessioned2026-07-07T05:52:09Z
dc.date.available2026-07-07T05:52:09Z
dc.descriptionWe describe a novel application of atomic force microscopy (AFM) to directly visualize cytoskeletal fibers in human foreskin epithelial cells. The nonionic detergent Triton X-100 in a low concentration was used to remove the membrane, soluble proteins, and organelles from the cell. The remaining cytoskeleton can then be directly visualized in either liquid or air-dried ambient conditions. These two types of scanning provide complimentary information. Scanning in liquid visualizes the surface filaments of the cytoskeleton, whereas scanning in air shows both the surface filaments and the total "volume" of the cytoskeletal fibers. The smallest fibers observed were ca. 50 nm in diameter. The lateral resolution of this technique was ca.20 nm, which can be increased to a single nanometer level by choosing sharper AFM tips. Because the AFM is a true three dimensional technique, we are able to quantify the observed cytoskeleton by its density and volume. The types of fibers can be identified by their size, similar to electron microscopy.
dc.description22 pages, PDF
dc.identifierhttps://arxiv.org/abs/physics/0406130
dc.identifierhttp://arxiv.org/abs/physics/0406130
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/86227
dc.subjectBiological Physics
dc.subjectInstrumentation and Detectors
dc.subjectMedical Physics
dc.subjectCell Behavior
dc.titleVisualization of Cytoskeletal Elements by the Atomic Force Microscope
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