Effects of Ox-LDL on Macrophages NAD(P)H Autofluorescence Changes by Two-photon Microscopy

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Ox-LDL uptakes by macrophage play a critical role in the happening of atherosclerosis. Because of its low damage on observed cells and better signal-to- background ratio, two-photon excitation fluorescence microscopy is used to observe NAD(P)H autofluorescence of macrophage under difference cultured conditions- bare cover glass, coated with fibronectin or poly-D-lysine. The results show that the optimal condition is fibronectin coated surface, on which, macrophages profile can be clearly identified on NAD(P)H autofluorescence images collected by two-photon microscopy. Moreover, different morphology and intensities of autofluorescence under different conditions were observed as well. In the future, effects of ox-LDL on macrophages will be investigated by purposed system to research etiology of atherosclerosis.
Submitted on behalf of TIMA Editions (http://irevues.inist.fr/tima-editions)

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Biological Physics

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http://salesiana.dossiersoluciones.com/handle/123456789/136050

Consulte el texto completo en el siguiente enlace:

https://arxiv.org/abs/0708.1849
 http://arxiv.org/abs/0708.1849
 Dans European Nano Systems Worshop - ENS 2005, Paris : France (2005)

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arXiv