Nanostructured Immunosensors. Application to the detection of Progesterone
| dc.creator | Pingarron, J. -M. | |
| dc.creator | Yanez-Sedeno, P. | |
| dc.creator | Gonzalez-Cortes, A. | |
| dc.creator | Carralero, V. | |
| dc.date | 2007-08-10 | |
| dc.date.accessioned | 2026-07-07T08:23:08Z | |
| dc.date.available | 2026-07-07T08:23:08Z | |
| dc.description | A novel nanostructured electrochemical immunsensor for the determination of progesterone is reported. The approach combines the properties of gold nanoparticles with the use of a graphite-Teflon composite electrode matrix, into which gold nanoparticles are incorporated by simple physical inclusion. The antibody anti-progesterone was directly attached to the electrode surface. The immunosensor functioning is based on competitive assay between progesterone and alkaline phosphatase-labelled progesterone. Monitoring of the affinity reaction was accomplished by the electrochemical oxidation of 1-naphtol. Modification of the graphite -Teflon electrode matrix with gold nanoparticles improves substantially the electrooxidation response of 1-naphtol. Using a detection potential of +0.3V, a detection limit for progesterone of 0.84 ng ml-1 was obtained. Analysis of seven milk samples spiked at a 3.5 ng ml-1 progesterone concentration level yielded a mean recovery of 101+6%. Detection of the antigen-antibody reaction with a graphite - Teflon - colloidal - gold - Tyrosinase electrode, using phenylphosphate as alkaline phosphatase substrate to generate phenol, which is subsequently reduced at -0.1 V at the composite electrode, produced a high improvement in the sensitivity for progesterone detection | |
| dc.description | Submitted on behalf of TIMA Editions (http://irevues.inist.fr/tima-editions) | |
| dc.identifier | https://arxiv.org/abs/0708.1456 | |
| dc.identifier | http://arxiv.org/abs/0708.1456 | |
| dc.identifier | Dans European Nano Systems Worshop - ENS 2006, Paris : France (2006) | |
| dc.identifier.uri | http://salesiana.dossiersoluciones.com/handle/123456789/135887 | |
| dc.subject | Materials Science | |
| dc.title | Nanostructured Immunosensors. Application to the detection of Progesterone | |
| dc.type | text |