Measurements of Multicomponent Diffusion Coefficients for Lysozyme Chloride in Water and Aqueous Na$_2$SO$_4$

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dc.creatorBuzatu, D.
dc.creatorPetrescu, E.
dc.creatorBuzatu, F. D.
dc.creatorAlbright, J. G.
dc.date2004-03-20
dc.date.accessioned2026-07-07T05:51:32Z
dc.date.available2026-07-07T05:51:32Z
dc.descriptionThis paper presents a diffusion experimental study for ternary lysozyme-Na$_2$SO$_4$-water system, from moderate precipitant concentrations into the supersaturated region and provides a complete set of four diffusion coefficients. These data are important in order to provide accurate models of protein diffusion with applications in growth of protein crystals for X-ray diffraction studies. All three-component mutual-diffusion experiments reported here were performed by Rayleigh interferometry at pH$=4.5$, T$=25^o$ C and at a mean lysozyme concentration (average of top and bottom solution concentrations) of 0.6 mM (8.6 mg/mL). Four experiments, with different combinations of protein and Na$_2$SO$_4$ concentration differences, were performed at each of five mean Na$_2$SO$_4$ concentrations (0.1, 0.25, 0.5, 0.65 and 0.8 M), for a total of 20 experiments. In addition, we have measured dynamic light-scattering diffusion coefficients of the ternary system lysozyme chloride-Na$_2$SO$_4$-water.
dc.description1 figure
dc.identifierhttps://arxiv.org/abs/physics/0403099
dc.identifierhttp://arxiv.org/abs/physics/0403099
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/86016
dc.subjectChemical Physics
dc.titleMeasurements of Multicomponent Diffusion Coefficients for Lysozyme Chloride in Water and Aqueous Na$_2$SO$_4$
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