From features to expression: High-density oligonucleotide array analysis revisited

dc.creatorNaef, Felix
dc.creatorLim, Daniel A.
dc.creatorPatil, Nila
dc.creatorMagnasco, Marcelo O.
dc.date2001-02-05
dc.date2001-02-21
dc.date.accessioned2026-07-07T05:45:25Z
dc.date.available2026-07-07T05:45:25Z
dc.descriptionOne of the most popular tools for large scale gene expression studies are high-density oligonucleotide (GeneChip(R)) arrays. These currently have 16-20 small probe cells (``features'') for evaluating the transcript abundance of each gene. In addition, each probe is accompanied by a mismatched probe designed as a control for non-specificity. An algorithm is presented to compute comparative expression levels from the intensities of the individual features, based on a statistical study of their distribution. Interestingly, MM probes need not be included in the analysis. We show that our algorithm improves significantly upon the current standard and leads to a substantially larger number of genes brought above the noise floor for further analysis.
dc.description9 pages, 7 figures
dc.identifierhttps://arxiv.org/abs/physics/0102010
dc.identifierhttp://arxiv.org/abs/physics/0102010
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/83985
dc.subjectBiological Physics
dc.subjectData Analysis, Statistics and Probability
dc.subjectQuantitative Biology
dc.titleFrom features to expression: High-density oligonucleotide array analysis revisited
dc.typetext

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