Silver Staining of Proteins in 2DE Gels

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dc.creatorLelong, Cécile
dc.creatorChevallet, Mireille
dc.creatorLuche, Sylvie
dc.creatorRabilloud, Thierry
dc.date2009-04-22
dc.date.accessioned2026-07-07T13:07:21Z
dc.date.available2026-07-07T13:07:21Z
dc.descriptionSilver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation, and finally image development. Several variants of silver staining are described here, which can be completed in a time range from 2 h to 1 day after the end of the electrophoretic separation. Once completed, the stain is stable for several weeks.
dc.identifierhttps://arxiv.org/abs/0904.3535
dc.identifierhttp://arxiv.org/abs/0904.3535
dc.identifierMethods in molecular biology (Clifton, N.J.) 519 (2009) 339-350
dc.identifierdoi:10.1007/978-1-59745-281-6_21
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/228063
dc.subjectGenomics
dc.titleSilver Staining of Proteins in 2DE Gels
dc.typetext

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