Protein Microarrays with Carbon Nanotubes as Multi-Color Raman Labels

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dc.creatorChen, Zhuo
dc.creatorTabakman, Scott M.
dc.creatorGoodwin, Andrew P.
dc.creatorKattah, Michael G.
dc.creatorDaranciang, Dan
dc.creatorWang, Xinran
dc.creatorZhang, Guangyu
dc.creatorLi, Xiaolin
dc.creatorLiu, Zhuang
dc.creatorUtz, Paul J.
dc.creatorJiang, Kaili
dc.creatorFan, Shoushan
dc.creatorDai, Hongjie
dc.date2008-10-31
dc.date.accessioned2026-07-07T10:14:38Z
dc.date.available2026-07-07T10:14:38Z
dc.descriptionDetection of biomolecules is important in proteomics and clinical diagnosis and treatment of diseases. Here, we apply functionalized, macromolecular, single walled carbon nanotubes SWNTs as multi-color Raman labels to protein arrays for highly sensitive, multiplexed protein detection. Raman detection utilizes the sharp peaks of SWNTs with minimal background interference, affording a high signal to noise ratio needed for ultra-sensitive detection. Surface-enhanced Raman scattering SERS combined with the strong resonance Raman intensity of SWNTs, affords detection sensitivity down to 1 fM, a three order of magnitude improvement over most of reported fluorescence-based protein detections. We show that human autoantibodies to Proteinase 3 aPR3, a biomarker for the autoimmune disease Wegeners granulomatosis, is detected by Raman in human serum up to a 107 dilution. Moreover, SWNT Raman tags are stable against photobleaching and quenching, and by conjugating different antibodies to pure 12C and 13C SWNT isotopes, we demonstrate two-color SWNT Raman-based protein detection in a multiplexed fashion.
dc.descriptionNature Biotechnology, in press
dc.identifierhttps://arxiv.org/abs/0810.5761
dc.identifierhttp://arxiv.org/abs/0810.5761
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/172949
dc.subjectMaterials Science
dc.titleProtein Microarrays with Carbon Nanotubes as Multi-Color Raman Labels
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