Fully denaturing two-dimensional electrophoresis of membrane proteins: a critical update

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dc.creatorRabilloud, Thierry
dc.creatorChevallet, Mireille
dc.creatorLuche, Sylvie
dc.creatorLelong, Cécile
dc.date2008-12-27
dc.date.accessioned2026-07-07T12:22:53Z
dc.date.available2026-07-07T12:22:53Z
dc.descriptionThe quality and ease of proteomics analysis depends on the performance of the analytical tools used, and thus of the performances of the protein separation tools used to deconvolute complex protein samples. Among protein samples, membrane proteins are one of the most difficult sample classes, because of their hydrophobicity and embedment in the lipid bilayers. This review deals with the recent progresses and advances made in the separation of membrane proteins by 2-DE separating only denatured proteins. Traditional 2-D methods, i.e., methods using IEF in the first dimension are compared to methods using only zone electrophoresis in both dimensions, i.e., electrophoresis in the presence of cationic or anionic detergents. The overall performances and fields of application of both types of method is critically examined, as are future prospects for this field.
dc.identifierhttps://arxiv.org/abs/0812.4736
dc.identifierhttp://arxiv.org/abs/0812.4736
dc.identifierProteomics 8, 19 (2008) 3965-73
dc.identifierdoi:10.1002/pmic.200800043
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/213804
dc.subjectGenomics
dc.titleFully denaturing two-dimensional electrophoresis of membrane proteins: a critical update
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