Micro-Bioreactor Mimicking a Cellular Environment

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dc.creatorHase, Masahiko
dc.creatorYamada, Ayako
dc.creatorHamada, Tsutomu
dc.creatorYoshikawa, Kenichi
dc.date2006-05-17
dc.date.accessioned2026-07-07T07:15:03Z
dc.date.available2026-07-07T07:15:03Z
dc.descriptionWe report that a cell-sized water droplet (CWD) with a diameter of several tens of microns can serve as a micro-biochemical reactor. Since the droplet inner surface is covered by a phospholipid layer, it provides an environment similar to that in living cells. The CWD is manipulated with laser tweezers and we monitor the time-development of biochemical reactions in a single CWD by fusing two droplets which contain a substrate and an enzyme, respectively. As actual examples of enzymatic reactions, we present results on the reaction of calcein with esterase and on the expression reaction of green fluorescent protein.
dc.identifierhttps://arxiv.org/abs/physics/0605145
dc.identifierhttp://arxiv.org/abs/physics/0605145
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/113125
dc.subjectBiological Physics
dc.titleMicro-Bioreactor Mimicking a Cellular Environment
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