Design Principle of Gene Expression Used by Human Stem Cells; Implication for Pluripotency

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dc.creatorGolan-Mashiach, Michal
dc.creatorDazard, Jean-Eudes
dc.creatorGerecht-Nir, Sharon
dc.creatorAmariglio, Ninette
dc.creatorFisher, Tamar
dc.creatorJacob-Hirsch, Jasmine
dc.creatorBielorai, Bella
dc.creatorOsenberg, Sivan
dc.creatorBarad, Omer
dc.creatorGetz, Gad
dc.creatorToren, Amos
dc.creatorRechavi, Gideon
dc.creatorEldor-Itskovitz, Joseph
dc.creatorDomany, Eytan
dc.creatorGivol, David
dc.date2004-09-15
dc.date.accessioned2026-07-07T05:58:38Z
dc.date.available2026-07-07T05:58:38Z
dc.descriptionHuman embryonic stem cells (ESC) are undifferentiated and are endowed with the capacities of self renewal and pluripotential differentiation. Adult stem cells renew their own tissue, but whether they can trans-differentiate to other tissues is still controversial. To understand the genetic program that underlies the pluripotency of stem cells, we compared the transcription profile of ESC with that of progenitor/stem cells of human hematopoietic and keratinocytic origins, along with their mature cells to be viewed as snapshots along tissue differentiation. ESC gene profile show higher complexity with significantly more highly expressed genes than adult cells. We hypothesize that ESC use a strategy of expressing genes that represent various differentiation pathways and selection of only a few for continuous expression upon differentiation to a particular target. Such a strategy may be necessary for the pluripotency of ESC. The progenitors of either hematopoietic or keratinocytic cells also follow the same design principle. Using advanced clustering, we show that many of the ESC expressed genes are turned off in the progenitors/stem cells followed by a further downregulation in adult tissues. Concomitantly, genes specific to the target tissue are upregulated towards matured cells of skin or blood.
dc.descriptionTo appear in FASEB Journal; 32 pages
dc.identifierhttps://arxiv.org/abs/q-bio/0409015
dc.identifierhttp://arxiv.org/abs/q-bio/0409015
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/88429
dc.subjectQuantitative Methods
dc.subjectOther Quantitative Biology
dc.titleDesign Principle of Gene Expression Used by Human Stem Cells; Implication for Pluripotency
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