Systematic identification of abundant A-to-I editing sites in the human transcriptome

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dc.creatorLevanon, Erez Y.
dc.creatorEisenberg, Eli
dc.creatorYelin, Rodrigo
dc.creatorNemzer, Sergey
dc.creatorHallegger, Martina
dc.creatorShemesh, Ronen
dc.creatorFligelman, Zipora Y.
dc.creatorShoshan, Avi
dc.creatorPollock, Sarah R.
dc.creatorSztybel, Dan
dc.creatorOlshansky, Moshe
dc.creatorRechavi, Gideon
dc.creatorJantsch, Michael F.
dc.date2004-11-25
dc.date.accessioned2026-07-07T05:58:46Z
dc.date.available2026-07-07T05:58:46Z
dc.descriptionRNA editing by members of the double-stranded RNA-specific ADAR family leads to site-specific conversion of adenosine to inosine (A-to-I) in precursor messenger RNAs. Editing by ADARs is believed to occur in all metazoa, and is essential for mammalian development. Currently, only a limited number of human ADAR substrates are known, while indirect evidence suggests a substantial fraction of all pre-mRNAs being affected. Here we describe a computational search for ADAR editing sites in the human transcriptome, using millions of available expressed sequences. 12,723 A-to-I editing sites were mapped in 1,637 different genes, with an estimated accuracy of 95%, raising the number of known editing sites by two orders of magnitude. We experimentally validated our method by verifying the occurrence of editing in 26 novel substrates. A-to-I editing in humans primarily occurs in non-coding regions of the RNA, typically in Alu repeats. Analysis of the large set of editing sites indicates the role of editing in controlling dsRNA stability.
dc.descriptionPre-print version. See http://dx.doi.org/10.1038/nbt996 for a reprint
dc.identifierhttps://arxiv.org/abs/q-bio/0411045
dc.identifierhttp://arxiv.org/abs/q-bio/0411045
dc.identifierNature Biotechnology 22, 1001-1005 (2004)
dc.identifierdoi:10.1038/nbt996
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/88484
dc.subjectGenomics
dc.titleSystematic identification of abundant A-to-I editing sites in the human transcriptome
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