Determination and Spectroscopy of Quantum Yields in Bio/Chemiluminescence via Novel Light-Collection-Efficiency Calibration: Reexamination of The Aqueous Luminol Chemiluminescence Standard

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dc.creatorAndo, Yoriko
dc.creatorNiwa, Kazuki
dc.creatorYamada, Nobuyuki
dc.creatorIrie, Tsutomu
dc.creatorEnomoto, Toshiteru
dc.creatorKubota, Hidehiro
dc.creatorOhmiya, Yoshihiro
dc.creatorAkiyama, Hidefumi
dc.date2006-10-17
dc.date.accessioned2026-07-07T07:30:10Z
dc.date.available2026-07-07T07:30:10Z
dc.descriptionWe have developed a luminescence-measurement system for liquid bio/chemiluminescence that can obtain quantitative luminescence spectra as the absolute total number of luminescence photons at each wavelength or photon energy and quantum yields. Calibration of light-collection efficiency in the system is performed with a reference double-plate cell. This method is applicable to sample cells of any kind suitable for measurement, which is a great advantage over previous techniques in practical experiments. Using this system, the quantum yield of aqueous luminol chemiluminescence was obtained as 1.23-+0.20%, which is in good agreement with previously reported values.
dc.description33 pages 6 figures 3 tables
dc.identifierhttps://arxiv.org/abs/q-bio/0610029
dc.identifierhttp://arxiv.org/abs/q-bio/0610029
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/118371
dc.subjectQuantitative Methods
dc.titleDetermination and Spectroscopy of Quantum Yields in Bio/Chemiluminescence via Novel Light-Collection-Efficiency Calibration: Reexamination of The Aqueous Luminol Chemiluminescence Standard
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