Micropropagation of three genotypes of Indian mustard [{Brassica juncea} (L.) Czern.] using seedling-derived transverse thin cell layer (tTCL) explants
| dc.creator | Aoun, Michel | |
| dc.creator | Charles, Gilbert | |
| dc.creator | Hourmant, Annick | |
| dc.date | 2008-07-07 | |
| dc.date.accessioned | 2026-07-07T09:48:51Z | |
| dc.date.available | 2026-07-07T09:48:51Z | |
| dc.description | Micropropagation of three genotypes of Indian mustard [\textit{Brassica juncea} (L.) Czern.] using 7-days old seedling-derived transverse thin cell layer (tTCL) explants was accomplished. The genotype, explant source and addition of silver nitrate to the medium significantly influenced shoot bud induction. MS medium with 26.6 $μ$M of 6-Benzylaminopurin (BAP) and 3.22 $μ$M of 1-naphtaleneacetic acid (NAA) was identical (in the case of cotyledon tTCLs whatever the organ) and superior for the induction of buds (in the cases of petiole tTCL explants of genotypes 1 and 2 and hypocotyl tTCL explants of genotypes 1 and 3) than 53.3 $μ$M of BAP and 3.22 $μ$M of NAA. However, 53.3 $μ$M of BAP was superior for the induction of buds than 26.6 $μ$M in the presence of the same concentration of NAA for petiole tTCL explants of genotype 3 and hypocotyl tTCL explants of genotype 2. The addition of silver nitrate significantly enhanced the rate of shoot induction in all genotypes. Cotyledon-derived tTCL explants exhibited the highest shoot bud induction potential and was followed by petiole- and hypocotyl-derived ones. Addition of 10 $μ$M of silver nitrate to BAP and NAA supplemented medium induced higher frequency shoot bud induction (up to 100 %) with the highest means of 4.45 shoots per cotyledon-derived tTCL explants obtained with the genotype 2. Shoot regenerated were rooted on MS basal medium without PGRs which induced 99 % of roots per shoot. The plantlets established in greenhouse conditions with 99 % survival, flowered normally and set seeds. | |
| dc.description | 12 pages, 2 figures and 2 tables | |
| dc.identifier | https://arxiv.org/abs/0807.1061 | |
| dc.identifier | http://arxiv.org/abs/0807.1061 | |
| dc.identifier.uri | http://salesiana.dossiersoluciones.com/handle/123456789/164367 | |
| dc.subject | Tissues and Organs | |
| dc.title | Micropropagation of three genotypes of Indian mustard [{Brassica juncea} (L.) Czern.] using seedling-derived transverse thin cell layer (tTCL) explants | |
| dc.type | text |