Spatial fluorescence cross correlation spectroscopy by means of a spatial light modulator

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dc.creatorBlancquaert, Yoann
dc.creatorGao, Jie
dc.creatorDerouard, Jacques
dc.creatorDelon, Antoine
dc.date2008-02-26
dc.date2008-06-13
dc.date.accessioned2026-07-07T09:44:07Z
dc.date.available2026-07-07T09:44:07Z
dc.descriptionSpatial Fluorescence Cross Correlation Spectroscopy is a rarely investigated version of Fluorescence Correlation Spectroscopy, in which the fluorescence signals from different observation volumes are cross-correlated. In the reported experiments, two observation volumes, typically shifted by a few $μ$m, are produced, with a Spatial Light Modulator and two adjustable pinholes. We illustrated the feasibility and potentiality of this technique by: i) measuring molecular flows, in the range 0.2 - 1.5 $μ$m/ms, of so-lutions seeded with fluorescent nanobeads or rhodamine molecules (simulating active transport phenomenons); ii) investigating the permeability of the phospholipidic membrane of Giant Unilamellar Vesicles versus hydrophilic or hydrophobic molecules (in that case the laser spots were set on both sides of the membrane). Theoretical descriptions are proposed together with a discus-sion about Fluorescence Correlation Spectroscopy based, alternative methods.
dc.description12 pages, 6 figures, 1 tableau
dc.identifierhttps://arxiv.org/abs/0802.3755
dc.identifierhttp://arxiv.org/abs/0802.3755
dc.identifierJ. Biophotonics (2008) 1-12
dc.identifierdoi:10.1002/jbio.200810506
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/162755
dc.subjectOptics
dc.titleSpatial fluorescence cross correlation spectroscopy by means of a spatial light modulator
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