Fast fluorescence dynamics in non-ratiometric calcium indicators

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A fluorescence decay of high-affinity non-ratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a new model for accurate measurements of the free Ca2+ concentration and dissociation constants of non-ratiometric calcium indicators.
3 pages, 2 figures, figures revised, added chi-square goodness of fit

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Biological Physics, Soft Condensed Matter

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http://salesiana.dossiersoluciones.com/handle/123456789/224405

Consulte el texto completo en el siguiente enlace:

https://arxiv.org/abs/0807.2756
 http://arxiv.org/abs/0807.2756
 Opt. Lett. 34 (2009) 362-364
 doi:10.1364/OL.34.000362

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arXiv