Sweet silver: A formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry

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dc.creatorChevallet, Mireille
dc.creatorLuche, Sylvie
dc.creatorDiemer, Hélène
dc.creatorStrub, Jean-Marc
dc.creatorVan Dorsselaer, Alain
dc.creatorRabilloud, Thierry
dc.date2008-12-20
dc.date.accessioned2026-07-07T12:21:08Z
dc.date.available2026-07-07T12:21:08Z
dc.descriptionProtein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness are also favored features. Silver staining combines many of these features, but its compatibility with MS is limited. We describe here, a new variant of silver staining that is completely formaldehyde-free. Reducing sugars in alkaline borate buffer are used as developers. While keeping the benefits of silver staining, this method is shown to afford a much better performance in terms of compatibility with MS, both in PMF by MALDI and in LC/ESI/MS/MS.
dc.identifierhttps://arxiv.org/abs/0812.3988
dc.identifierhttp://arxiv.org/abs/0812.3988
dc.identifierProteomics 8, 23-24 (2008) 4853-61
dc.identifierdoi:10.1002/pmic.200800321
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/213269
dc.subjectGenomics
dc.titleSweet silver: A formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry
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