Study the effects of metallic ions on the combination of DNA and histones with molecular combing technique

Simple item page
dc.creatorLiu, Yu-Ying
dc.creatorWang, Peng-Ye
dc.creatorDou, Shuo-Xing
dc.creatorXie, Ping
dc.creatorWang, Wei-Chi
dc.creatorYin, Hua-Wei
dc.date2005-03-07
dc.date.accessioned2026-07-07T05:59:08Z
dc.date.available2026-07-07T05:59:08Z
dc.descriptionThe effects of monovalent (Na+, K+) and divalent (Mg2+, Ca2+, Mn2+) ions on the interaction between DNA and histone are studied using the molecular combing technique. Lamda-DNA molecules and DNA-histone complexes incubated with metal cations (Na+, K+, Mg2+, Ca2+, Mn2+) are stretched on hydrophobic surfaces, and directly observed by fluorescence microscopy. The results indicate that when these cations are added into the DNA solution, the fluorescence intensities of the stained DNA are reduced differently. The monovalent cations (Na+, K+) inhibit binding of histone to DNA. The divalent cations (Mg2+, Ca2+, Mn2+) enhance significantly the binding of histone to DNA and the binding of the DNA-histone complex to the hydrophobic surface. Mn2+ also induces condensation and aggregation of the DNA-histone complex.
dc.description11 pages, 7 figures
dc.identifierhttps://arxiv.org/abs/q-bio/0503012
dc.identifierhttp://arxiv.org/abs/q-bio/0503012
dc.identifierChinese Science Bulletin, Vol.50, No. 8 (2005) 731-737
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/88570
dc.subjectBiomolecules
dc.titleStudy the effects of metallic ions on the combination of DNA and histones with molecular combing technique
dc.typetext

Files

Collections

arXiv