On the lipid-bacterial protein interaction studied by quartz crystal microbalance with dissipation, transmission electron microscopy and atomic force microscopy

Thumbnail Image

Date

Authors

Journal Title

Journal ISSN

Volume Title

Publisher

Abstract

Description

The interaction between the bacterial S-protein SbpA on different types of lipid membranes has been studied using atomic force microscopy, transmission electron microscopy, and quartz crystal microbalance with dissipation. On one hand, It has been found that the bacterial forms two dimensional nanocrystals on zwitterionic DOPC bilayers and negatively charged DMPG vesicles adsorbed on mica, on zwitterionic DPPC and charged DPPC/DMPG (1:1) monolayers adsorbed on carbon grids. On the other hand, SbpA protein adsorption took place on zwitterionic DOPC bilayers and DOPC/DOPS (4:1) bilayers, previously adsorbed on silicon supports. SbpA adsorption also took place on DPPC/DOPS (1:1) monolayers adsorbed on carbon grids. Finally, neither SbpA adsorption, nor recrystallization was observed on zwitterionic DMPC vesicles (previously adsorbed on polyelectrolyte multilayers), and on DPPC vesicles supported on silicon.
30 pages, 20 figures

Keywords

Biological Physics

Citation

URI

http://salesiana.dossiersoluciones.com/handle/123456789/226444

Consulte el texto completo en el siguiente enlace:

https://arxiv.org/abs/0904.1662
 http://arxiv.org/abs/0904.1662

Collections

arXiv