Structural plasticity of single chromatin fibers revealed by torsional manipulation

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dc.creatorBancaud, Aurelien
dc.creatorSilva, Natalia Conde e
dc.creatorBarbi, Maria
dc.creatorWagner, Gaudeline
dc.creatorAllemand, Jean-Francois
dc.creatorMozziconacci, Julien
dc.creatorLavelle, Christophe
dc.creatorCroquette, Vincent
dc.creatorVictor, Jean-Marc
dc.creatorPrunell, Ariel
dc.creatorViovy, Jean-Louis
dc.date2007-07-13
dc.date.accessioned2026-07-07T08:21:16Z
dc.date.available2026-07-07T08:21:16Z
dc.descriptionMagnetic tweezers are used to study the mechanical response under torsion of single nucleosome arrays reconstituted on tandem repeats of 5S positioning sequences. Regular arrays are extremely resilient and can reversibly accommodate a large amount of supercoiling without much change in length. This behavior is quantitatively described by a molecular model of the chromatin 3-D architecture. In this model, we assume the existence of a dynamic equilibrium between three conformations of the nucleosome, which are determined by the crossing status of the entry/exit DNAs (positive, null or negative). Torsional strain, in displacing that equilibrium, extensively reorganizes the fiber architecture. The model explains a number of long-standing topological questions regarding DNA in chromatin, and may provide the ground to better understand the dynamic binding of most chromatin-associated proteins.
dc.description18 pages, 7 figures, Supplementary information available at http://www.nature.com/nsmb/journal/v13/n5/suppinfo/nsmb1087_S1.html
dc.identifierhttps://arxiv.org/abs/0707.2047
dc.identifierhttp://arxiv.org/abs/0707.2047
dc.identifierNature Structural and Molecular Biology 13, 444-450, 2006
dc.identifierdoi:10.1038/nsmb1087
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/135302
dc.subjectBiomolecules
dc.subjectBiological Physics
dc.subjectSubcellular Processes
dc.titleStructural plasticity of single chromatin fibers revealed by torsional manipulation
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