Shoot regeneration seedlings from transverse thin cell layer explants excised from cotyledon, petiole, hypocotyl of [{Brassica juncea} L. Czern.] in the presence of CdCl$_2$
| dc.creator | Aoun, Michel | |
| dc.creator | Cabon, Jean-Yves | |
| dc.creator | Hourmant, Annick | |
| dc.date | 2008-07-07 | |
| dc.date.accessioned | 2026-07-07T09:48:50Z | |
| dc.date.available | 2026-07-07T09:48:50Z | |
| dc.description | The present work describes a novel neoformation process of \textit{Brassica juncea} (L.) Czern. seedlings from transverse thin cell layers in the presence of CdCl$_2$. In order to investigate the regeneration ability of this crop, the effect of CdCl$_2$ on shoot regeneration (frequency of regeneration and bud number per tTCL) was examined. The tTCL explants were excised from cotyledon, petiole and hypocotyl of 7 day-old \textit{B. juncea} seedlings and cultivated on a solid basal MS medium supplemented with $α$-naphtalenacetic acid (NAA : 3.22 $μ$M), 6-benzylamino-purine (BAP : 26.6 $μ$M), sucrose (2 %, w/v), silver nitrate (AgNO3 : 10 $μ$M) and various concentrations of CdCl$_2$ (0 - 250 $μ$M). A concentration of CdCl$_2$ of 5 $μ$M is enough to reduce significantly the percentage of regenerated tTCL from 95-100 % to 77-86 % for all organs tested. In addition, 5 $μ$M of CdCl$_2$ reduces the bud number from 4.5 to 2.98 and 3.2 to 2.02 of hypocotyl and petiole tTCLs, but not for cotyledon tTCLs. Besides, 250 $μ$M of CdCl$_2$ is lethal for all tTCLs whatever the organ, and 200 $μ$M is lethal only for cotyledon and petiole tTCLs but not for hypocotyl explants which had 3.6 % of frequency of shoot regeneration. Plantlets regenerated from all shoots, whatever the treatment, developed and flowered normally 6 weeks after the transfer to pots. The regenerated plants were fertile and identical to source plants. | |
| dc.description | 12 pages, 2 figures and 3 tables | |
| dc.identifier | https://arxiv.org/abs/0807.1055 | |
| dc.identifier | http://arxiv.org/abs/0807.1055 | |
| dc.identifier.uri | http://salesiana.dossiersoluciones.com/handle/123456789/164365 | |
| dc.subject | Cell Behavior | |
| dc.title | Shoot regeneration seedlings from transverse thin cell layer explants excised from cotyledon, petiole, hypocotyl of [{Brassica juncea} L. Czern.] in the presence of CdCl$_2$ | |
| dc.type | text |