Determination of apparent activating energy of solubilizing MLV-DPPC and MLV-egg-yolk lecithin with surfactants by rectangular optic diffusimetry
Abstract
Description
It were determinated the apparent activating energy of dipalmitoylphosphatidylcholine (DPPC) multilamellar liposome (MLV) solubilizing with sodium deoxycholate (DOCNa) by turbidi-metric measurements and the apparent activating energy of egg-yolk lecithin (EYL)- MLV solubilizing with different surfactants (DOCNa, TX-100, CTMB) by rectangular optic diffu-si-met-ric measurements. The apparent activating energy of MLV-DPPC solubilizing with DOCNa is Ea = 127.7kJ/mol. The apparent activating energy of MLV-EYL solubilizing with cetyl-trimet-hylamonium bromide (CTMB) is 22.3kJ/mol and with DOCNa is 47.3kJ/mol (average value) and with Triton X-100 is 99.3kJ/mol. On the basis of the above data it can be said that besides the utilisation of the liposomes in the modelling of cellular membranes, they can be used as experimental models, too, in order to investigate the solubilization capacity of surfactants.