Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses

Simple item page
dc.creatorScheuber, Anita
dc.creatorRudge, Rachel
dc.creatorDanglot, Lydia
dc.creatorRaposo, Graca
dc.creatorBinz, Thomas
dc.creatorPoncer, Jean-Christophe
dc.creatorGalli, Thierry
dc.date2006-10-30
dc.date.accessioned2026-07-07T07:30:13Z
dc.date.available2026-07-07T07:30:13Z
dc.descriptionSynaptic vesicle (SV) exocytosis mediating neurotransmitter release occurs spontaneously at low intraterminal calcium concentrations and is stimulated by a rise in intracellular calcium. Exocytosis is compensated for by the reformation of vesicles at plasma membrane and endosomes. Although the adaptor complex AP-3 was proposed to be involved in the formation of SVs from endosomes, whether its function has an indirect effect on exocytosis remains unknown. Using mocha mice, which are deficient in functional AP-3, we identify an AP-3-dependent tetanus neurotoxin-resistant asynchronous release that can be evoked at hippocampal mossy fiber (MF) synapses. Presynaptic targeting of the tetanus neurotoxin-resistant vesicle soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) is lost in mocha hippocampal MF terminals, whereas the localization of synaptobrevin 2 is unaffected. In addition, quantal release in mocha cultures is more frequent and more sensitive to sucrose. We conclude that lack of AP-3 results in more constitutive secretion and loss of an asynchronous evoked release component, suggesting an important function of AP-3 in regulating SV exocytosis at MF terminals.
dc.identifierhttps://arxiv.org/abs/q-bio/0610056
dc.identifierhttp://arxiv.org/abs/q-bio/0610056
dc.identifierProc Natl Acad Sci U S A (20/10/2006) sous presse
dc.identifierdoi:10.1073/pnas.0603511103
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/118388
dc.subjectNeurons and Cognition
dc.subjectSubcellular Processes
dc.titleLoss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses
dc.typetext

Files

Collections

arXiv