Biodegradation of 2-ethylhexyl nitrate (2-EHN) by Mycobacterium austroafricanum IFP 2173

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dc.creatorNicolau, Elodie
dc.creatorKerhoas, L.
dc.creatorLettere, Martine
dc.creatorJouanneau, Yves
dc.creatorMarchal, Rémy
dc.date2009-04-23
dc.date.accessioned2026-07-07T13:07:54Z
dc.date.available2026-07-07T13:07:54Z
dc.description2-Ethyhexyl nitrate (2-EHN) is a major additive of fuel which is used to comply with the cetane number of diesel. Because of its wide use and possible accidental release, 2-EHN is a potential pollutant of the environment. In this study, Mycobacterium austroafricanum IFP 2173 was selected among several strains as the best 2-EHN degrader. The 2-EHN biodegradation rate was increased in biphasic cultures where the hydrocarbon was dissolved in an inert non-aqueous phase liquid (NAPL), suggesting that the transfer of the hydrophobic substrate to the cells was a growth-limiting factor. Carbon balance calculation as well as organic carbon measurement indicated a release of metabolites in the culture medium. Further analysis by gas chromatography revealed that a single metabolite accumulated during growth. This metabolite had a molecular mass of 114 Da as determined by GC/MS and was provisionally identified as 4-ethyldihydrofuran-2(3H)-one by LC-MS/MS analysis. Identification was confirmed by analysis of the chemically synthesized lactone. Based on these results, a plausible catabolic pathway is proposed whereby 2-EHN is converted to 4-ethyldihydrofuran-2(3H)-one, which cannot be metabolised further by strain IFP 2173. This putative pathway provides an explanation for the low energetic efficiency of 2-EHN degradation and its poor biodegradability.
dc.identifierhttps://arxiv.org/abs/0904.3649
dc.identifierhttp://arxiv.org/abs/0904.3649
dc.identifierApplied and Environmental Microbiology 74 (2008) 6187-6193
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/228245
dc.subjectBiomolecules
dc.titleBiodegradation of 2-ethylhexyl nitrate (2-EHN) by Mycobacterium austroafricanum IFP 2173
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