Fluorescence microscopy of single autofluorescent proteins for cellular biology

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dc.creatorCognet, Laurent
dc.creatorCoussen, Françoise
dc.creatorChoquet, Daniel
dc.creatorLounis, Brahim
dc.date2007-04-29
dc.date.accessioned2026-07-07T07:58:42Z
dc.date.available2026-07-07T07:58:42Z
dc.descriptionIn this paper we review the applicability of autofluorescent proteins for single-molecule imaging in biology. The photophysical characteristics of several mutants of the Green Fluorescent Protein (GFP) and those of DsRed are compared and critically discussed for their use in cellular biology. The alternative use of two-photon excitation at the single-molecule level or Fluorescence Correlation Spectroscopy is envisaged for the study of individual autofluorescent proteins. Single-molecule experiments performed in live cells using eGFP and preferably eYFP fusion proteins are reviewed. Finally, the first use at the single-molecule level of citrine, a more photostable variant of the eYFP is reported when fused to a receptor for neurotransmitter in live cells.
dc.identifierhttps://arxiv.org/abs/0704.3853
dc.identifierhttp://arxiv.org/abs/0704.3853
dc.identifierComptes Rendus de l Académie des Sciences - Series IV - Physics 3 (15/08/2002) 645
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/128101
dc.subjectOptics
dc.subjectBiological Physics
dc.titleFluorescence microscopy of single autofluorescent proteins for cellular biology
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