2-Aminopurine fluorescence quenching and lifetimes: Role of base stacking

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dc.creatorJean, John M.
dc.creatorHall, Kathleen B.
dc.date2000-12-19
dc.date.accessioned2026-07-07T01:46:02Z
dc.date.available2026-07-07T01:46:02Z
dc.description2-Aminopurine (2AP) is a fluorescent analog of guanosine and adenosine and has been used to probe nucleic acid structure and dynamics. Its spectral features in nucleic acids have been interpreted phenomenologically, in the absence of a rigorous electronic description of the context-dependence of 2AP fluorescence. Now, by using time-dependent density functional theory, we describe the excited-state properties of 2AP in a B-form dinucleotide stacked with guanosine, adenosine, cytosine, or thymine. Calculations predict that 2AP fluorescence is quenched statically when stacked with purines, because of mixing of the molecular orbitals in the ground state. In contrast, quenching is predicted to be dynamic when 2AP is stacked with pyrimidines, because of formation of a low-lying dark excited state. The different quenching mechanisms will result in different experimentally measured fluorescence lifetimes and quantum yields.
dc.identifierhttps://pmc.ncbi.nlm.nih.gov/articles/PMC14540/
dc.identifierhttps://pubmed.ncbi.nlm.nih.gov/11120885/
dc.identifierhttps://doi.org/10.1073/pnas.011442198
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/154
dc.languageen
dc.publisherNational Academy of Sciences
dc.rightsCopyright © 2001, The National Academy of Sciences
dc.sourceProc Natl Acad Sci U S A
dc.subjectBiological Sciences
dc.title2-Aminopurine fluorescence quenching and lifetimes: Role of base stacking
dc.typeText

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