Functioning of the dimeric GABA(B) receptor extracellular domain revealed by glycan wedge scanning

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dc.creatorRondard, Philippe
dc.creatorHuang, Siluo
dc.creatorMonnier, Carine
dc.creatorTu, Haijun
dc.creatorBlanchard, Bertrand
dc.creatorOueslati, Nadia
dc.creatorMalhaire, Fanny
dc.creatorLi, Ying
dc.creatorTrinquet, Eric
dc.creatorLabesse, Gilles
dc.creatorPin, Jean-Philippe
dc.creatorLiu, Jianfeng
dc.date2008-09-10
dc.date.accessioned2026-07-07T10:01:58Z
dc.date.available2026-07-07T10:01:58Z
dc.descriptionThe G-protein-coupled receptor (GPCR) activated by the neurotransmitter GABA is made up of two subunits, GABA(B1) and GABA(B2). GABA(B1) binds agonists, whereas GABA(B2) is required for trafficking GABA(B1) to the cell surface, increasing agonist affinity to GABA(B1), and activating associated G proteins. These subunits each comprise two domains, a Venus flytrap domain (VFT) and a heptahelical transmembrane domain (7TM). How agonist binding to the GABA(B1) VFT leads to GABA(B2) 7TM activation remains unknown. Here, we used a glycan wedge scanning approach to investigate how the GABA(B) VFT dimer controls receptor activity. We first identified the dimerization interface using a bioinformatics approach and then showed that introducing an N-glycan at this interface prevents the association of the two subunits and abolishes all activities of GABA(B2), including agonist activation of the G protein. We also identified a second region in the VFT where insertion of an N-glycan does not prevent dimerization, but blocks agonist activation of the receptor. These data provide new insight into the function of this prototypical GPCR and demonstrate that a change in the dimerization interface is required for receptor activation.
dc.identifierhttps://arxiv.org/abs/0809.1716
dc.identifierhttp://arxiv.org/abs/0809.1716
dc.identifierThe EMBO Journal 27, 9 (2008) 1321-1332
dc.identifierdoi:10.1038/emboj.2008.64
dc.identifier.urihttp://salesiana.dossiersoluciones.com/handle/123456789/168811
dc.subjectNeurons and Cognition
dc.subjectBiomolecules
dc.titleFunctioning of the dimeric GABA(B) receptor extracellular domain revealed by glycan wedge scanning
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