2026-07-072026-07-07http://salesiana.dossiersoluciones.com/handle/123456789/219006Power spectral density measurements of any sampled signal are typically restricted by both acquisition rate and frequency response limitations of instruments, which can be particularly prohibitive for video-based measurements. We have developed a new method called Intensity Modulation Spectral Analysis (IMSA) that circumvents these limitations, dramatically extending the effective detection bandwidth. We demonstrate this by video-tracking an optically-trapped microsphere while oscillating an LED illumination source. This approach allows us to quantify fluctuations of the microsphere at frequencies over 10 times higher than the Nyquist frequency, mimicking a significantly higher frame rate.4 pages, 2 figuresBiological PhysicsData Analysis, Statistics and ProbabilityInstrumentation and DetectorsBeyond the frame rate: Measuring high-frequency fluctuations with light intensity modulationtext