2026-07-072026-07-07http://salesiana.dossiersoluciones.com/handle/123456789/88351We present the results of an exact analysis of a model energy landscape of a protein to clarify the notion of the transition state and the physical meaning of the $ϕ$ values determined in protein engineering experiments. We benchmark our findings to various theoretical approaches proposed in the literature for the identification and characterization of the transition state.Protein Science in pressBiomoleculesStatistical MechanicsWhat one can learn from experiments about the elusive transition state?text